Identifying and characterising insect integrated membrane proteins
“Identifying novel and efficacious insect control traits is a key challenge for the entire agricultural industry. A significant decision point for a new trait is determining if we have found a novel mode-of-action. To improve our decisions, we are looking for faster and more efficient methods to allow us to characterize and differentiate binding sites of our candidate proteins. I’m excited to see what new methodologies are being developed for other applications and in other industries that can accelerate our agricultural product development. Advancements here will help Syngenta more rapidly develop technologies and products that growers across the globe need.”
Syngenta is seeking technologies or services that can identify and characterize surface and/or integrated membrane proteins in insect midgut membranes that specifically interact with insecticidal proteins in order to screen for new leads and assess their mode of action.
Ideally the solution will allow this process to be completed quickly and at a high throughput. Novel methods of quantitatively assessing specific interaction between insecticidal protein and midgut membrane are of interest.
The specific binding of insecticidal proteins to receptors located in the brush border of insect midgut epithelial cells is known to be the key step that leads to insecticidal activity. Insecticidal proteins can also bind to the brush border membrane in non-specific and non-productive ways. However, specific binding to the brush border membrane is an essential step. Therefore, distinguishing specific vs non-specific binding is critical.
Possible solution areas include, but are not limited to, membrane protein purification with or without membrane lipids, solubilized membrane protein preparations, high-throughput technologies to quantify binding of labelled or non-labelled proteins to membranes and cells, and the characterization of protein binding via lipids, sugars, and protein receptors.
If you're working on solutions and approaches across industries such as pharmaceuticals, biotech, medical sciences, chemicals/reagents, in addition to solutions currently used in agricultural applications, then you're likely to be a good fit for this challenge.
We've a wide range of potential collaboration types, including with academic research groups, start-ups, and small to medium-sized companies, as well as organizations that perform these services.
Closing date:31 May 2024
What we're looking for
- High confidence in identifying specific protein:receptor interactions (reduce false positives)
- High sensitivity to characterize protein:receptor affinity
- High throughput binding evaluations
- Ideally have experience with proteins binding to membrane receptors.
- Experience working with insect sourced membranes
What we can offer you
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